Objectives
- to re-make the Au/BSA solutions, because the mixtures from yesterday did not react,
- to re-suspend the fibers in varying concentrations of Tris Buffer with pHs 10 and 8, and
- to run UV-vis on the resuspended fibers.
Description
Adjustments:
- The spatula was wrapped in parafilm to measure out both the BSA and the HAuCl4.
- Only one concentration was made initially as a test. It was placed in the oven at 85°C for an hour to make sure a reaction was taking place. This TEST sample was removed after an hour. Dark Blue/Purple fibers formed. This TEST sample was saved for later testing.
- After it was confirmed that a reaction was indeed taking place, the mixtures were made with concentrations of 60μM, 80μM, 100μM, 120μM, 128μM, 130μM, 132μM, 133μM, 134μM, 136μM, 138μM, 140μM, 160μM, and 170μM, taking into account the new masses and new actual concentrations of the stock solutions.
Adjustments:
- 1mL of the resuspended fibers were examined.
Data
- Table of Measurements for Au/BSA mixtures
| Stock Solution Concentrations
|
'
|
'
|
'
|
'
|
| Theoretical |
|
|
Actual [uM] |
|
| BSA |
HAuCl4 |
|
BSA |
HAuCl4
|
| 15 uM |
10 mM |
|
15.355 |
10501
|
| Vol [μL]
|
'
|
| BSA |
HAuCl4
|
| 4000 |
4000
|
| μM Ratio [Au/BSA]
|
'
|
Volume
|
'
|
'
|
| BSA |
HAuCl4 |
BSA [μL] |
HAuCl4 [μL] |
H2O [mL]
|
| 1 |
60 |
260.5014653 |
157.515 |
3.581983535
|
| 1 |
80 |
260.5014653 |
210.02 |
3.529478535
|
| 1 |
100 |
260.5014653 |
262.525 |
3.476973535
|
| 1 |
120 |
260.5014653 |
315.03 |
3.424468535
|
| 1 |
128 |
260.5014653 |
336.032 |
3.403466535
|
| 1 |
130 |
260.5014653 |
341.2825 |
3.398216035
|
| 1 |
132 |
260.5014653 |
346.533 |
3.392965535
|
| 1 |
133 |
260.5014653 |
349.15825 |
3.390340285
|
| 1 |
134 |
260.5014653 |
351.7835 |
3.387715035
|
| 1 |
136 |
260.5014653 |
357.034 |
3.382464535
|
| 1 |
138 |
260.5014653 |
362.2845 |
3.377214035
|
| 1 |
140 |
260.5014653 |
367.535 |
3.371963535
|
| 1 |
160 |
260.5014653 |
420.04 |
3.319458535
|
| 1 |
170 |
260.5014653 |
446.2925 |
3.293206035
|
|
|
|
4623.06525 |
Total μL
|
- UV-Vis of Resuspended Fibers via Tris Buffer
Notes
- The stocks were made with the incorrect concentrations, so the reaction did not occur properly.
Image of solutions made September 5th
- All the solutions from the batch made on September 5th and a close-up of the solutions from the batch made on September 5th. The solution was made with the parafilmed spatula. THe solution spent 4 hours in a 85°C oven.
|
AU Biomaterials Design Lab
