User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/05: Difference between revisions
From OpenWetWare
(→Data) |
Dhea Patel (talk | contribs) |
||
Line 21: | Line 21: | ||
#After it was confirmed that a reaction was indeed taking place, the mixtures were made with concentrations of 60μM, 80μM, 100μM, 120μM, 128μM, 130μM, 132μM, 133μM, 134μM, 136μM, 138μM, 140μM, 160μM, and 170μM, taking into account the new masses and new actual concentrations of the stock solutions. | #After it was confirmed that a reaction was indeed taking place, the mixtures were made with concentrations of 60μM, 80μM, 100μM, 120μM, 128μM, 130μM, 132μM, 133μM, 134μM, 136μM, 138μM, 140μM, 160μM, and 170μM, taking into account the new masses and new actual concentrations of the stock solutions. | ||
*[[User:Melissa_Novy/Notebook/CHEM-571/2012/09/05| | *Re-suspending Fibers | ||
* | **Please refer to [[User:Melissa_Novy/Notebook/CHEM-571/2012/09/05| Melissa's entry]] for details on centrifuging the Au/BSA solutions and adding the Tris buffer. | ||
***2 different pHs of Tris buffer were tested: pH 8 and 10. | |||
***The concentrations of each pH of Tris buffer was observed using 1 mM, 100 μM, and 10 μM Tris buffer on fibers with arbitrary AU/BSA molar ratios. | |||
*[[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/09/04| UV-vis]] | *[[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/09/04| UV-vis]] | ||
**UV-Vis was run on 1mL of the resuspended fibers, with the wavelength range of 700-200nm, looking for a peak around 550nm. | |||
**The data was plotted on an Absorbance vs. Wavelength graph. | |||
* | |||
==Data== | ==Data== |
Revision as of 11:16, 25 September 2012
AU Biomaterials Design Lab | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Objectives
DescriptionAdjustments:
Data
Notes
Image of solutions made September 5th
|