User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/04

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Contents

Objective

  • to run UV-Vis spectra on the Au/BSA mixtures that Dr. Miller made on Friday,
  • to centrifuge the mixtures that Dr. Miller made,
  • to make 100mM Tris Buffer with pH 10 and pH 8, and
  • to remake Au/BSA mixtures with concentrations of 60-133 because no purple solutions were created in the other mixtures.

Description

  • UV-Vis
  1. UV-Vis was run on 1mL samples of water and each sample with the wavelength range of 700-200nm, looking for a peak around 550nm.
  2. The data points were collected and imported in Excel.
  3. The data was corrected for water and plotted to better visualize the absorbance.
  • Remaking of the Au/BSA Solutons
  1. The Au/BSA solutions were remade using the same protocol from last week, except that the metal spatula was wrapped in parafilm so that the metal from the spatula would not react with the gold. Concentrations of 60, 80, 100, 120, 128, 130, 132, and 133 were made.

Data and Calculations

  • Table of Measurements for Au/BSA mixtures
Stock Solution Concentrations ' ' ' '
TheoreticalActual [uM]
BSAHAuCl4BSAHAuCl4
15 uM10 mM15.8069862
Vol [uL] '
BSAHAuCl4
40004000
uM Ratio [Au/BSA] ' Volume [uL] ' '
BSAHAuCl4BSA [μL]HAuCl4H2O [mL]
160253.07147.933.5990
180253.07197.243.5497
1100253.07246.553.5004
1120253.07295.863.4511
1128253.07315.583.4313
1130253.07320.523.4264
1132253.07325.453.4215
1133253.07327.913.4190
1134253.07330.383.4166
1136253.07335.313.4116
1138253.07340.243.4067
1140253.07345.173.4018
1160253.07394.483.3525
1170253.07419.143.3278
4341.75Total uL

Notes

  • Only the mixture with concentration of 80 Au/BSA was a homologous purple mixture. [made 8/30]
  • The stocks made today were made with the incorrect concentrations, so the reaction did not occur properly.

Images from the Solutions made by Dr. Miller

Image:Dr_Miller_Solutions.jpg

  • All the solutions from Dr Miller's batch from August 30. The solution was made with the parafilmed spatula. The solution spent 4 hours in a 85°C oven.


Image of Solution made on September 4th

Image:IMAG0730.jpg

  • All the solutions looked identical. The reactants did not react with each other, and the concentration ratios were off significantly.
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