User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/04: Difference between revisions
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*'''Remaking of the Au/BSA Solutons''' | *'''Remaking of the Au/BSA Solutons''' | ||
#The Au/BSA solutions were remade using the same protocol from [ | #The Au/BSA solutions were remade using the same protocol from [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/08/29| last week]], except that the metal spatula was wrapped in parafilm so that the metal from the spatula would not react with the gold. Concentrations of 60, 80, 100, 120, 128, 130, 132, and 133 were made. | ||
==Data and Calculations== | ==Data and Calculations== | ||
*'''Table of Measurements for Au/BSA mixtures''' | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Stock Solution Concentrations''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
|- | |||
| Theoretical||||||Actual [uM]|| | |||
|- | |||
| BSA||HAuCl4||||BSA||HAuCl4 | |||
|- | |||
| 15 uM||10 mM||||15.806||9862 | |||
|} | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Vol [uL]''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
|- | |||
| BSA||HAuCl4 | |||
|- | |||
| 4000||4000 | |||
|} | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''uM Ratio [Au/BSA]''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume [uL]''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
|- | |||
| BSA||HAuCl4||BSA [μL]||HAuCl4||H2O [mL] | |||
|- | |||
| 1||60||253.07||147.93||3.5990 | |||
|- | |||
| 1||80||253.07||197.24||3.5497 | |||
|- | |||
| 1||100||253.07||246.55||3.5004 | |||
|- | |||
| 1||120||253.07||295.86||3.4511 | |||
|- | |||
| 1||128||253.07||315.58||3.4313 | |||
|- | |||
| 1||130||253.07||320.52||3.4264 | |||
|- | |||
| 1||132||253.07||325.45||3.4215 | |||
|- | |||
| 1||133||253.07||327.91||3.4190 | |||
|- | |||
| 1||134||253.07||330.38||3.4166 | |||
|- | |||
| 1||136||253.07||335.31||3.4116 | |||
|- | |||
| 1||138||253.07||340.24||3.4067 | |||
|- | |||
| 1||140||253.07||345.17||3.4018 | |||
|- | |||
| 1||160||253.07||394.48||3.3525 | |||
|- | |||
| 1||170||253.07||419.14||3.3278 | |||
|- | |||
| ||||||4341.75||Total uL | |||
|} | |||
==Notes== | ==Notes== | ||
Only the mixture with concentration of 80 Au/BSA was a homologous purple mixture. | *Only the mixture with concentration of 80 Au/BSA was a homologous purple mixture. [made 8/30] | ||
*The stocks made today were made with the incorrect concentrations, so the reaction did not occur properly. | |||
==Images from the Solutions made by Dr. Miller== | |||
[[Image:Dr_Miller_Solutions.jpg]] | |||
*All the solutions from Dr Miller's batch from August 30. The solution was made with the parafilmed spatula. The solution spent 4 hours in a 85°C oven. | |||
==Image of Solution made on September 4th== | |||
[[Image:IMAG0730.jpg]] | |||
*All the solutions looked identical. The reactants did not react with each other, and the concentration ratios were off significantly. |
Revision as of 11:54, 12 September 2012
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Objective
Description
Data and Calculations
Notes
Images from the Solutions made by Dr. Miller
Image of Solution made on September 4th
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