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|style="background-color: #EEE"|[[Image: | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|300px]]<span style="font-size:22px;"> AU Biomaterials Design Lab</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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==Objective== | |||
*to run UV-Vis spectra on the Au/BSA mixtures that Dr. Miller made on [[User:Abigail_E._Miller/Notebook/CHEM571_Lab_Project/2012/08/31| Friday]], | |||
*to centrifuge the mixtures that Dr. Miller made, | |||
*to make 100mM Tris Buffer with pH 10 and pH 8, and | |||
*to remake Au/BSA mixtures with concentrations of 60-133 because no purple solutions were created in the other mixtures. | |||
==Description== | |||
*'''UV-Vis''' | |||
#UV-Vis was run on 1mL samples of water and each sample with the wavelength range of 700-200nm, looking for a peak around 550nm. | |||
#The data points were collected and imported in Excel. | |||
#The data was corrected for water and plotted to better visualize the absorbance. | |||
*'''[[User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/09/04| Description of Centrifuge and Observations]]''' | |||
*'''[[User:Melissa_Novy/Notebook/CHEM-571/2012/09/04| Preparation of Tris Buffer]]''' | |||
*'''Remaking of the Au/BSA Solutons''' | |||
#The Au/BSA solutions were remade using the same protocol from [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/08/29| last week]], except that the metal spatula was wrapped in parafilm so that the metal from the spatula would not react with the gold. Concentrations of 60, 80, 100, 120, 128, 130, 132, and 133 were made. | |||
==Data and Calculations== | |||
*'''Table of Measurements for Au/BSA mixtures''' | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Stock Solution Concentrations''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
|- | |||
| Theoretical||||||Actual [uM]|| | |||
|- | |||
| BSA||HAuCl4||||BSA||HAuCl4 | |||
|- | |||
| 15 uM||10 mM||||15.806||9862 | |||
|} | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Vol [uL]''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
|- | |||
| BSA||HAuCl4 | |||
|- | |||
| 4000||4000 | |||
|} | |} | ||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''uM Ratio [Au/BSA]''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume [uL]''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
|- | |||
| BSA||HAuCl4||BSA [μL]||HAuCl4||H2O [mL] | |||
|- | |||
| 1||60||253.07||147.93||3.5990 | |||
|- | |||
| 1||80||253.07||197.24||3.5497 | |||
|- | |||
| 1||100||253.07||246.55||3.5004 | |||
|- | |||
| 1||120||253.07||295.86||3.4511 | |||
|- | |||
| 1||128||253.07||315.58||3.4313 | |||
|- | |||
| 1||130||253.07||320.52||3.4264 | |||
|- | |||
| 1||132||253.07||325.45||3.4215 | |||
|- | |||
| 1||133||253.07||327.91||3.4190 | |||
|- | |||
| 1||134||253.07||330.38||3.4166 | |||
|- | |||
| 1||136||253.07||335.31||3.4116 | |||
|- | |||
| 1||138||253.07||340.24||3.4067 | |||
|- | |||
| 1||140||253.07||345.17||3.4018 | |||
|- | |||
| 1||160||253.07||394.48||3.3525 | |||
|- | |||
| 1||170||253.07||419.14||3.3278 | |||
|- | |||
| ||||||4341.75||Total uL | |||
|} | |||
==Notes== | |||
*Only the mixture with concentration of 80 Au/BSA was a homologous purple mixture. [made 8/30] | |||
*The stocks made today were made with the incorrect concentrations, so the reaction did not occur properly. | |||
==Images from the Solutions made by Dr. Miller== | |||
[[Image:Dr_Miller_Solutions.jpg]] | |||
*All the solutions from Dr Miller's batch from August 30. The solution was made with the parafilmed spatula. The solution spent 4 hours in a 85°C oven. | |||
==Image of Solution made on September 4th== | |||
[[Image:IMAG0730.jpg]] | |||
*All the solutions looked identical. The reactants did not react with each other, and the concentration ratios were off significantly. |
Revision as of 11:54, 12 September 2012
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Objective
Description
Data and Calculations
Notes
Images from the Solutions made by Dr. Miller
Image of Solution made on September 4th
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