User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/04: Difference between revisions
From OpenWetWare
Dhea Patel (talk | contribs) |
Dhea Patel (talk | contribs) No edit summary |
||
Line 21: | Line 21: | ||
*'''UV-Vis''' | *'''UV-Vis''' | ||
#UV-Vis was run on 1mL samples of water and each sample with the wavelength range of 700-200nm, looking for a peak around 550nm. | |||
#The data points were collected and imported in Excel. | |||
#The data was corrected for water and plotted to better visualize the absorbance. | |||
*'''[http://openwetware.org/wiki/User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/09/04 Description of Centrifuge and Observations]''' | *'''[http://openwetware.org/wiki/User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/09/04 Description of Centrifuge and Observations]''' | ||
Line 30: | Line 30: | ||
*'''Remaking of the Au/BSA Solutons''' | *'''Remaking of the Au/BSA Solutons''' | ||
#The Au/BSA solutions were remade using the same protocol from [http://openwetware.org/wiki/User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/08/29 last week], except that the metal spatula was wrapped in parafilm so that the metal from the spatula would not react with the gold. Concentrations of 60, 80, 100, 120, 128, 130, 132, and 133 were made. | #The Au/BSA solutions were remade using the same protocol from [http://openwetware.org/wiki/User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/08/29 last week], except that the metal spatula was wrapped in parafilm so that the metal from the spatula would not react with the gold. Concentrations of 60, 80, 100, 120, 128, 130, 132, and 133 were made. | ||
==Data and Calculations== | ==Data and Calculations== |
Revision as of 19:52, 4 September 2012
Phosphorylation Lab | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Objective
Description
#UV-Vis was run on 1mL samples of water and each sample with the wavelength range of 700-200nm, looking for a peak around 550nm. #The data points were collected and imported in Excel. #The data was corrected for water and plotted to better visualize the absorbance.
#The Au/BSA solutions were remade using the same protocol from last week, except that the metal spatula was wrapped in parafilm so that the metal from the spatula would not react with the gold. Concentrations of 60, 80, 100, 120, 128, 130, 132, and 133 were made. Data and CalculationsN/A NotesOnly the mixture with concentration of 80 Au/BSA was a homologous purple mixture. |