User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/04: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:Hartings_AU_Phosphorylation_Header.png|250px]]<span style="font-size:22px;"> Phosphorylation Lab</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
* Insert content here...




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__NOTOC__
 
==Objective==
 
*to run UV-Vis spectra on the Au/BSA mixtures that Dr. Miller made on [http://openwetware.org/wiki/User:Abigail_E._Miller/Notebook/CHEM571_Lab_Project/2012/08/31 Friday],
*to centrifuge the mixtures that Dr. Miller made,
*to make 100mM Tris Buffer with pH 10 and pH 8, and
*to remake Au/BSA mixtures with concentrations of 60-133 because no purple solutions were created in the other mixtures.
 
 
==Description==
 
*'''UV-Vis'''
1. UV-Vis was run on 1mL samples of water and each sample with the wavelength range of 700-200nm, looking for a peak around 550nm.
2. The data points were collected and imported in Excel.
3. The data was corrected for water and plotted to better visualize the absorbance.
 
*[User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/09/04 Description of Centrifuge and Observations]
 
*[User:Melissa_Novy/Notebook/CHEM-571/2012/09/04 Preparation of Tris Buffer]
 
*The Au/BSA solutions were remade using the same protocol from [User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/08/29 last week], except that the metal spatula was wrapped in parafilm so that the metal from the spatula would not react with the gold. Concentrations of 60, 80, 100, 120, 128, 130, 132, and 133 were made.
 
==Data and Calculations==
 
N/A
 
==Notes==
Only the mixture with concentration of 80 Au/BSA was a homologous purple mixture.

Revision as of 19:48, 4 September 2012

Phosphorylation Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>



Objective

  • to run UV-Vis spectra on the Au/BSA mixtures that Dr. Miller made on Friday,
  • to centrifuge the mixtures that Dr. Miller made,
  • to make 100mM Tris Buffer with pH 10 and pH 8, and
  • to remake Au/BSA mixtures with concentrations of 60-133 because no purple solutions were created in the other mixtures.


Description

  • UV-Vis

1. UV-Vis was run on 1mL samples of water and each sample with the wavelength range of 700-200nm, looking for a peak around 550nm. 2. The data points were collected and imported in Excel. 3. The data was corrected for water and plotted to better visualize the absorbance.

  • [User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/09/04 Description of Centrifuge and Observations]
  • [User:Melissa_Novy/Notebook/CHEM-571/2012/09/04 Preparation of Tris Buffer]
  • The Au/BSA solutions were remade using the same protocol from [User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/08/29 last week], except that the metal spatula was wrapped in parafilm so that the metal from the spatula would not react with the gold. Concentrations of 60, 80, 100, 120, 128, 130, 132, and 133 were made.

Data and Calculations

N/A

Notes

Only the mixture with concentration of 80 Au/BSA was a homologous purple mixture.

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