User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/08/29: Difference between revisions
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==Notes== | ==Notes== | ||
**The stocks were made with the incorrect concentrations, so the reaction did not occur properly. | **The stocks were made with the incorrect concentrations, so the reaction did not occur properly. | ||
**The stocks were made without the parafilm protection on the spatula. | |||
==Images of the Solutions Produced== | |||
[[Image:20120829_60AuBSA.jpg]] | |||
*The 60 Au/BSA from August 29. The solution was made without the parafilmed spatula. THe solution spent 4 hours in a 85°C oven. | |||
[[Image:20120829_80120AuBSA.jpg]] | |||
*The 80 Au/BSA, 100 Au/BSA, 120 Au/BSA, and 128 Au/BSA solutions from August 29. The solution was made without the parafilmed spatula. THe solution spent 4 hours in a 85°C oven. | |||
[[Image:20120829_130134AuBSA.jpg]] | |||
*The 130 Au/BSA, 132 Au/BSA, 133 Au/BSA, and 134 Au/BSA solutions from August 29. The solution was made without the parafilmed spatula. THe solution spent 4 hours in a 85°C oven. | |||
[[Image:20120829_136170AuBSA.jpg]] | |||
*The 136 Au/BSA, 138 Au/BSA, 140 Au/BSA, 160 Au/BSA, and 170 Au/BSA solutions from August 29. The solution was made without the parafilmed spatula. THe solution spent 4 hours in a 85°C oven. |
Revision as of 07:37, 12 September 2012
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Objective
Description
Data and Calculations
FW HAuCl4: 393.83 g/mol Theoretical Concentration of Stock: 10mM Volume of Stock: 25mL Theoretical Mass: 25mL × 10-3 L/mL × 10mM × 10-3 mol/mmol × 393.83 g/mol = 0.0985 g HAuCl4 Measured Mass: 0.0971 g HAuCl4 Calculated Concentration: 9862 μM 0.0971g × (1/393.83 g/mol) × (1/25E-3 L) × 106 μM/mol = 9862 μM
FW BSA: 66430 g/mol Theoretical Concentration of Stock: 15μM Volume of Stock: 10mL Theoretical Mass: 10mL × 10-3 L/mL × 15μM × 10-6 mol/mmol × 66430 g/mol = 0.00996 g BSA Measured Mass: 0.0105 g BSA Calculated Concentration: 15.806 μM 0.0105g × (1/66430 g/mol) × (1/10E-3 L) × 106 μM/mol = 15.806 μM
Notes
Images of the Solutions Produced
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