User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/04/10: Difference between revisions
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Mary Mendoza (talk | contribs) (Autocreate 2013/04/10 Entry for User:Dhea_Patel/Notebook/CHEM_572:_ADA&Inhibitor_Kinetics) |
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== | ==Objective== | ||
* | * Taken from [[User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/04/10|Mary Mendoza]]. | ||
* Using solutions made last week, a run of 40 μM adenosine with 25 μM varying inhibitors were conducted using the UV 2500 Shimadzu spectrophotometer. | |||
* Three runs of each inhibitor were taken; the average of the runs were taken. The standard deviation were calculated and added as error bars. | |||
* Produce histogram | |||
==Protocol== | |||
* The reagents were added in the following sequence: | |||
** 1.78 mL of phosphate buffer | |||
** 600 μL of 200 μM adenosine (final concentration 50 μM) | |||
* This was allowed to mix through venting and placed on the chamber for absorbance reading. | |||
** 300 μL of phosphate buffer (or inhibitor dissolved in phosphate buffer) | |||
** 300 μL of dimethyl sulfoxide (DMSO) (or inhibitor dissolved in DMSO) | |||
* The cuvette contents were mixed by venting and placed on the chamber for absorbance reading. | |||
** 20 μL of adenosine deaminase (ADA) was added to catalyze the reaction | |||
* The kinetics of the reaction was taken using the UVProbe software. | |||
==Description== | |||
*Cuvette Mixture with Inhibitor | |||
[[Image:InhibitorCuvetteTable.png]] | |||
*Cuvette Mixture no Inhibitor | |||
[[Image:CuvetteMixtureNoInhibitor.png]] | |||
*Inhibitors Ran today: | |||
#Quercetin | |||
#Myricetin | |||
==Data== | |||
[[Image:410.png]] | |||
[[Image:410pc.png]] | |||
==Notes== | |||
*Solution Preparation/Calculations: Catherine Koenigsknecht | |||
*Cuvette preparation: Mary Mendoza | |||
*Data Evaluation: Dhea Patel | |||
*Overall Lab Assistance: Mike Nagle | |||
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Revision as of 23:55, 11 April 2013
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Objective
Protocol
Description
DataNotes
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