User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/04/10

From OpenWetWare

< User:Dhea Patel | Notebook | CHEM 572: ADA&Inhibitor Kinetics | 2013 | 04(Difference between revisions)
Jump to: navigation, search
(Objective)
Current revision (17:18, 8 May 2013) (view source)
(Notes)
 
(4 intermediate revisions not shown.)
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-
==Objective==
 
-
* Taken from [[User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/04/10|Mary Mendoza]].
 
-
* Using solutions made last week, a run of 40 μM adenosine with 50 μM varying inhibitors were conducted using the UV 2500 Shimadzu spectrophotometer.
 
-
* Three runs of each inhibitor were taken; the average of the runs were taken. The standard deviation were calculated and added as error bars.
 
-
* Produce histogram
 
-
==Protocol==
 
-
* The reagents were added in the following sequence:
 
-
** 1.78 mL of phosphate buffer
 
-
** 600 μL of 200 μM adenosine (final concentration 50 μM)
 
-
* This was allowed to mix through venting and placed on  the chamber for absorbance reading.
 
-
** 300 μL of phosphate buffer (or inhibitor dissolved in phosphate buffer)
 
-
** 300 μL of dimethyl sulfoxide (DMSO) (or inhibitor dissolved in DMSO)
 
-
* The cuvette contents were mixed by venting and placed on the chamber for absorbance reading.
 
-
** 20 μL of adenosine deaminase (ADA) was added to catalyze the reaction
 
-
* The kinetics of the reaction was taken using the UVProbe software.
 
-
==Description==
 
-
*Cuvette Mixture with Inhibitor
 
-
[[Image:InhibitorCuvetteTable.png]]
 
-
*Cuvette Mixture no Inhibitor
 
-
[[Image:CuvetteMixtureNoInhibitor.png]]
 
-
*Inhibitors Ran today:
 
-
#Quercetin
 
-
#Myricetin
 
-
==Data==
 
-
[[Image:410.png]]
 
-
[[Image:410pc.png]]
 
-
==Notes==
 
-
*Solution Preparation/Calculations: Catherine Koenigsknecht
 
-
*Cuvette preparation: Mary Mendoza
 
-
*Data Evaluation: Dhea Patel
 
-
*Overall Lab Assistance: Mike Nagle
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Current revision

Project name Main project page
Previous entry      






Personal tools