User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/04/09

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(Objective)
(Protocol)
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==Protocol==
 
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* The reagents were added in the following sequence:
 
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** 1.78 mL of phosphate buffer
 
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** 600 μL of 200 μM adenosine (final concentration 50 μM)
 
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* This was allowed to mix through venting and placed on  the chamber for absorbance reading.
 
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** 300 μL of phosphate buffer (or inhibitor dissolved in phosphate buffer)
 
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** 300 μL of dimethyl sulfoxide (DMSO) (or inhibitor dissolved in DMSO)
 
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* The cuvette contents were mixed by venting and placed on the chamber for absorbance reading.
 
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** 20 μL of adenosine deaminase (ADA) was added to catalyze the reaction
 
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* The kinetics of the reaction was taken using the UVProbe software.
 
==Description==
==Description==

Revision as of 17:16, 8 May 2013

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Description

  • Cuvette Mixture with Inhibitor

Image:InhibitorCuvetteTable.png

  • Cuvette Mixture no Inhibitor

Image:CuvetteMixtureNoInhibitor.png

  • Inhibitors Ran today:
  1. Aspirin
  2. Kaempferol

Data

  • Image:409HistogramTable.png
  • Image:409AverageVelocityHisto.png
  • Image:409PercentChangeHisto.png

Notes

  • Solution Preparation/Calculations: Catherine Koenigsknecht
  • Cuvette preparation: Mary Mendoza
  • Data Evaluation: Dhea Patel
  • Overall Lab Assistance: Mike Nagle



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