User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/04/03

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Preparation of Reagents

  • 500 μM of each inhibitor was made with newly made 0.05 M phosphate buffer solution at the pH of 7.4.
  • A new stock solution of 200 μM adenosine stock solution was also prepared.
  • For the kinetic assay runs, it was decided to revert back to the original, chosen adenosine concentration of 40 μM.
  • Adenosine trials were performed at 265 nm. A histogram of that trial is shown below.



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