User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/02/27: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
mNo edit summary
Line 21: Line 21:
[[Image:Screen_Shot_2013-02-27_at_2.57.04_PM.png]]
[[Image:Screen_Shot_2013-02-27_at_2.57.04_PM.png]]


==Data==
==Data from [Inhibitor] = 0==


[[Image:20130227_ADA_Kinetics_3T.tiff|px200]]
[[Image:20130227_ADA_Kinetics_3T.tiff|px200]]
Line 29: Line 29:
[[Image:20130227_average_ADA_Kinetics_3T.tiff|px200]]
[[Image:20130227_average_ADA_Kinetics_3T.tiff|px200]]


==Data from [Inhibitor] = 50uM==
[[Image:Screen_Shot_2013-03-05_at_12.03.39_PM.png]]


<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 10:06, 5 March 2013

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objective

  • to begin ADA + Inhibitor kinetics

Description

  • 150uM Acetylsalicylic Acid Inhibitor stock solution (in Phosphate buffer) was made using the following calculations:

  • 25 and 40uM with [Inhibitor] = 0 was re-run for Trial 3.
  • More 0.05M phosphate buffer was prepared according to the procedure outlined in January's entry.
  • ADA kinetics in the presence of the Acetylsalicylic Acid was started using the following table:

Data from [Inhibitor] = 0

px200

px200

px200

Data from [Inhibitor] = 50uM


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Dhea_Patel/Notebook/CHEM_572:_ADA%26Inhibitor_Kinetics/2013/02/27&oldid=680823"