User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/02/12
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| - | == | + | ==Objective== |
| - | * | + | * to run ADA kinetics assay |
| + | ==Methods== | ||
| + | *Baseline 200-400nm using pH7.4, 0.005M phosphate buffer | ||
| + | *Temperature control: 25°C | ||
| + | *Phosphate buffer in reference cell | ||
| + | *Ran kinetics on 2.56uM solution (20uL ADA, 600uL 12.8uM Adenosine, and 2.38mL pH7.4, 0.005M phosphate buffer) for 5 minutes at 265nm. | ||
| + | * | ||
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Revision as of 13:27, 12 February 2013
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