User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/02/12

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(Autocreate 2013/02/12 Entry for User:Dhea_Patel/Notebook/CHEM_572:_ADA&Inhibitor_Kinetics)
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==Entry title==
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==Objective==
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* Insert content here...
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* to run ADA kinetics assay
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==Methods==
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*Baseline 200-400nm using pH7.4, 0.005M phosphate buffer
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*Temperature control: 25°C
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*Phosphate buffer in reference cell
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*Ran kinetics on 2.56uM solution (20uL ADA, 600uL 12.8uM Adenosine, and 2.38mL pH7.4, 0.005M phosphate buffer) for 5 minutes at 265nm.
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*
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Revision as of 12:27, 12 February 2013

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Objective

  • to run ADA kinetics assay

Methods

  • Baseline 200-400nm using pH7.4, 0.005M phosphate buffer
  • Temperature control: 25°C
  • Phosphate buffer in reference cell
  • Ran kinetics on 2.56uM solution (20uL ADA, 600uL 12.8uM Adenosine, and 2.38mL pH7.4, 0.005M phosphate buffer) for 5 minutes at 265nm.


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