User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/02/12: Difference between revisions
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== | ==Objective== | ||
* | * to run ADA kinetics assay | ||
==Methods== | |||
*Baseline 200-400nm using pH7.4, 0.005M phosphate buffer | |||
*Temperature control: 25°C | |||
*Phosphate buffer in reference cell | |||
*Ran kinetics on 2.56uM solution (20uL ADA, 600uL 12.8uM Adenosine, and 2.38mL pH7.4, 0.005M phosphate buffer) for 5 minutes at 265nm. | |||
* | |||
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Revision as of 10:27, 12 February 2013
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