User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/02/05: Difference between revisions
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**The absorbance was close to 0, indicating that the ideal adenosine concentration likely ranges between 10μM and 100μM. | **The absorbance was close to 0, indicating that the ideal adenosine concentration likely ranges between 10μM and 100μM. | ||
*Then, an assay was run at 265nm using 2.38mL phosphate buffer, 600μL of 5μM stock adenosine (to make a final concentration of 1μM), and 20μL ADA (as outlined in the table above). | *Then, an assay was run at 265nm using 2.38mL phosphate buffer, 600μL of 5μM stock adenosine (to make a final concentration of 1μM), and 20μL ADA (as outlined in the table above). | ||
*From the spectra obtained today, the following tables were created: | |||
**This table is the volumes of 5mM adenosine and phosphate buffer used to make the new adenosine stock solutions. | |||
***[[Image:Screen_Shot_2013-02-05_at_9.01.20_PM.png]] | |||
**This table is the updated table of the volumes and concentrations of solutions that will be added in the cuvette. | |||
***[[Image:Screen_Shot_2013-02-05_at_9.01.33_PM.png]] | |||
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Revision as of 19:07, 5 February 2013
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Objective
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