User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/02/05: Difference between revisions

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**Note that the table has been adjusted based on the change in concentration of the adenosine stock solution.  
**Note that the table has been adjusted based on the change in concentration of the adenosine stock solution.  
[[Image:Screen_Shot_2013-02-05_at_11.59.42_AM.png]]
[[Image:Screen_Shot_2013-02-05_at_11.59.42_AM.png]]
*The kinetics was run using UV-vis at 25°C at 265nm for 5 minutes.  
*The kinetics was run using UV-vis at 25°C at 265nm for 5 minutes.


==Procedure==
==Procedure==

Revision as of 10:51, 5 February 2013

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Objective

  • to perform ADA kinetics assays using UV-vis.

Calculations

  • ADA Kinetics were run using the following volumes and concentrations.
    • Note that the table has been adjusted based on the change in concentration of the adenosine stock solution.

  • The kinetics was run using UV-vis at 25°C at 265nm for 5 minutes.

Procedure

  1. UVProbe was opened.
    1. Window > 1. Kinetics
    2. Methods Icon
      1. Wavelength: 265nm
      2. Duration: 600 seconds (5minutes)
      3. OK
  2. Shimadzu CPS-Controller was set to 25°C.
    1. wait for the temperature to raise to 25°C
  3. place the sample in the cell and click start.

Observations


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