User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/01/29: Difference between revisions
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==Description== | ==Description== | ||
1. Reallocating ADA | 1. Reallocating ADA | ||
* | *Measure 2.5mg was added to a tube and stored at 4°C | ||
**0.5U/(19.8U/mg) = 0.02525mg | **0.5U/(19.8U/mg) = 0.02525mg | ||
2. 0.05M Sodium Phosphate Buffer (pH 7.4) | |||
*Add 3.1 g of NaH2PO4•H2O and 10.9 g of Na2HPO4 (anhydrous) to distilled H2O to make a volume of 1 L, 1M Sodium Phosphate Buffer. | |||
**The pH of the solution was 7.03, so 1M NaOH was added drop-wise until the solution reached a pH of 7.4. | |||
**This buffer was stored at 4°C. | |||
*50mL of the 1M Sodium Phosphate Buffer was diluted in 1L H<sub>2</sub>O. | |||
3. Preparing a stock solution of adenosine | |||
*Initially, 0.0802g of adenosine was added to 10mL of 0.05M Sodium Phosphate Buffer (to make 30mM solution), however the solution was super saturated. | |||
*Then, 0.0401g of adenosine was added to 10mL of 0.05M Sodium Phosphate Buffer (to make 15mM solution), but the solution was still saturated. | |||
**5mL of 0.05M Sodium Phosphate Buffer was added to this solution in order (to make 10mM solution), but one flake of adenosine remained out of solution. | |||
**5mL of 0.05M Sodium Phosphate Buffer was added to the 10mM solution (to make 5mM solution) and the adenosine was fully dissolved in solution. | |||
*The final concentration of the adenosine stock solution was 5mM. | |||
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Revision as of 09:52, 30 January 2013
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Objective
Description1. Reallocating ADA
2. 0.05M Sodium Phosphate Buffer (pH 7.4)
3. Preparing a stock solution of adenosine
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