User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/01/23

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'''Protocol for 0.1 M Sodium Phosphate Buffer (pH 7.4)'''
'''Protocol for 0.1 M Sodium Phosphate Buffer (pH 7.4)'''
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*Add 3.1 g of NaH2PO4•H2O and 10.9 g of Na2HPO4 (anhydrous) to distilled H2O to make a volume of 1 L.  
+
*Add 3.1 g of NaH<sub>2</sub>PO<sub>4</sub>•H<sub>2</sub>O and 10.9 g of Na<sub>2</sub>HPO<sub>4</sub> (anhydrous) to distilled H<sub>2</sub>O to make a volume of 1 L.  
**The pH of the final solution will be 7.4.  
**The pH of the final solution will be 7.4.  
**This buffer can be stored for up to 1 mo at 4°C.
**This buffer can be stored for up to 1 mo at 4°C.
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*Dilute to 0.05M: (take 50 mL of 1M solution and dilute in 1 Liter H2O)
+
*Dilute to 0.05M: (take 50 mL of 1M solution and dilute in 1 Liter H<sub>2</sub>O)
'''Running ADA Kinetics'''
'''Running ADA Kinetics'''

Revision as of 14:15, 29 January 2013

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Objective

  • To complete project proposal
  • To complete experimental protocol for next week.


Experimental Protocol

Protocol for 0.1 M Sodium Phosphate Buffer (pH 7.4)

  • Add 3.1 g of NaH2PO4•H2O and 10.9 g of Na2HPO4 (anhydrous) to distilled H2O to make a volume of 1 L.
    • The pH of the final solution will be 7.4.
    • This buffer can be stored for up to 1 mo at 4°C.
  • Dilute to 0.05M: (take 50 mL of 1M solution and dilute in 1 Liter H2O)

Running ADA Kinetics

  • The following table outlines the concentrations and volumes of the solutions used in the ADA kinetics assay to be performed next Tuesday.

px80

  • To prepare a 30 mM stock solution of adenosine:

\frac{0.030mol}{L} of adenosine × \frac{267.24  g}{1  mol} = \frac{8.0172g}{L} of adenosine in buffer

  • To make 10mL of solution, 0.080172g of adenosine will be added to 10mL of phosphate buffer.



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