User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/01/23

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|colspan="2" style="background-color: #F2F2F2;" align="right"|[[{{FULLPAGENAME}}/Entry_Base|Customize your entry pages]] [[Help:Notebook/Project_Base/Customize_entry_page|<html><img src="/images/a/aa/Help.png" border="0" /></html>]]
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==Entry title==
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* Insert content here...
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==Objective==
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*To complete project proposal
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*To complete experimental protocol for next week.
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==Experimental Protocol==
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'''Protocol for 0.1 M Sodium Phosphate Buffer (pH 7.4)'''
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*Add 3.1 g of NaH2PO4•H2O and 10.9 g of Na2HPO4 (anhydrous) to distilled H2O to make a volume of 1 L.
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**The pH of the final solution will be 7.4.
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**This buffer can be stored for up to 1 mo at 4°C.
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*Dilute to 0.05M: (take 50 mL of 1M solution and dilute in 1 Liter H2O)
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'''Running ADA Kinetics'''
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*The following table outlines the concentrations and volumes of the solutions used in the ADA kinetics assay to be performed [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2013/01/29| next Tuesday]].
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[[Image:ADA_kinetics_table.png|px80]]
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*To prepare a 30 mM stock solution of adenosine:
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<math>\frac{0.030mol}{L}</math> of adenosine × <math>\frac{267.24  g}{1  mol}</math> = <math>\frac{8.0172g}{L}</math> of adenosine in buffer
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*To make 10mL of solution, 0.080172g of adenosine will be added to 10mL of phosphate buffer.
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Revision as of 16:28, 23 January 2013

Search this Project

Customize your entry pages

Objective

  • To complete project proposal
  • To complete experimental protocol for next week.


Experimental Protocol

Protocol for 0.1 M Sodium Phosphate Buffer (pH 7.4)

  • Add 3.1 g of NaH2PO4•H2O and 10.9 g of Na2HPO4 (anhydrous) to distilled H2O to make a volume of 1 L.
    • The pH of the final solution will be 7.4.
    • This buffer can be stored for up to 1 mo at 4°C.
  • Dilute to 0.05M: (take 50 mL of 1M solution and dilute in 1 Liter H2O)

Running ADA Kinetics

  • The following table outlines the concentrations and volumes of the solutions used in the ADA kinetics assay to be performed next Tuesday.

px80

  • To prepare a 30 mM stock solution of adenosine:

\frac{0.030mol}{L} of adenosine × \frac{267.24 g}{1 mol} = \frac{8.0172g}{L} of adenosine in buffer

  • To make 10mL of solution, 0.080172g of adenosine will be added to 10mL of phosphate buffer.



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