User:David K. Barclay/Notebook/Controlling Pancreas Cell Fate Using Transcription Factors/2015/02/26: Difference between revisions
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(Autocreate 2015/02/26 Entry for User:David_K._Barclay/Notebook/Controlling_Pancreas_Cell_Fate_Using_Transcription_Factors) |
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== | ==RT-PCR== | ||
* | * Testing primers DB07-DB13 against cDNA06 | ||
* Followed [http://openwetware.org/wiki/Haynes:SYBRGreen Sybr Green RT-PCR Protocol] | |||
==Pancreas Cell== | |||
* Passage by cell split protocol | |||
* Cells at 70% confluency in T-25 starter medium | |||
* Deactivate 2mL of trypsin with 4mL of DMEM/20% FBS (6mL cells) | |||
* Transfer 3mL of cells into 7mL of DMEM/20% FBS (2 T75 flasks) | |||
* Two T75 flasks of TC1-alpha Pancreatic cells in 37 incubator | |||
Revision as of 10:33, 27 February 2015
 Cell Fate Switch by Synthetic Transcription Factors
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RT-PCR
Pancreas Cell
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