User:David K. Barclay/Notebook/Controlling Pancreas Cell Fate Using Transcription Factors/2015/02/26: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Cell Fate Switch by Synthetic Transcription Factors</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Cell Fate Switch by Synthetic Transcription Factors</span>
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* Testing primers DB07-DB13 against cDNA06
* Testing primers DB07-DB13 against cDNA06
* Followed [http://openwetware.org/wiki/Haynes:SYBRGreen Sybr Green RT-PCR Protocol]
* Followed [http://openwetware.org/wiki/Haynes:SYBRGreen Sybr Green RT-PCR Protocol]
* 7 Primers (primer and template master mixes different)


==Pancreas Cell==
==Pancreas Cell==

Latest revision as of 00:47, 27 September 2017

Cell Fate Switch by Synthetic Transcription Factors Main project page
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RT-PCR

  • Testing primers DB07-DB13 against cDNA06
  • Followed Sybr Green RT-PCR Protocol
  • 7 Primers (primer and template master mixes different)

Pancreas Cell

  • Passage by cell split protocol
  • Cells at 70% confluency in T-25 starter medium
  • Deactivate 2mL of trypsin with 4mL of DMEM/20% FBS (6mL cells)
  • Transfer 3mL of cells into 7mL of DMEM/20% FBS (2 T75 flasks)
  • Two T75 flasks of TC1-alpha Pancreatic cells in 37 incubator




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