User:David K. Barclay/Notebook/Controlling Pancreas Cell Fate Using Transcription Factors/2014/09/17: Difference between revisions
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(Autocreate 2014/09/17 Entry for User:David_K._Barclay/Notebook/Controlling_Pancreas_Cell_Fate_Using_Transcription_Factors) |
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Cell Fate Switch by Synthetic Transcription Factors</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Cell Fate Switch by Synthetic Transcription Factors</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==Transfer Thawed (0), TC1Alpha(2)== | ||
* | * 65% confluent T-25 flask. Cells passaged from thawed (9/5). | ||
* Transfer cells into one well in a 6 well plate. | |||
* Used Protocol: [http://openwetware.org/wiki/Haynes:SplittingCells Splitting Cells] | |||
* Volumes changed: 2 mL PBS, 0.5 mL Trypsin, 3.5 mL DMEM. | |||
* 4 mL transferred into 1 well. | |||
* Cells to be grown in 10cm dishes after confluence with well. | |||
Latest revision as of 00:18, 27 September 2017
Cell Fate Switch by Synthetic Transcription Factors | Main project page Previous entry Next entry |
Transfer Thawed (0), TC1Alpha(2)
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