User:David K. Barclay/Notebook/Controlling Pancreas Cell Fate Using Transcription Factors/2014/07/10: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Cell Fate Switch by Synthetic Transcription Factors</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Cell Fate Switch by Synthetic Transcription Factors</span>
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==Entry title==
==Immunocytology T1==
* Insert content here...
[http://openwetware.org/wiki/Haynes:Mamalian_IFC Standard Procedure: Immunocytology]
 
Antibodies Used:
{| {{table}}
|-
| Antibody || Secondary/Primary || Fluorophore || Dilution || Catalog
|-
| Glucagon (FL-180) || Primary || --- || 1:500 || sc-13091
|-
| Insulin  || Primary || --- || 1:250 || ab7842
|-
| Goat Anti-Guinea Pig IgG || Secondary || Red-Alexaflour 594 || 1:1000 || ab1501888
|-
| Rabbit Anti-Glucagon || Secondary || Green || 1:1000 || ---
|-
| Hoechst 33325 || Secondary || Blue || 1:1000 || ---
|}
 
===Results===
*Too few cells fixated for useful results. Plates were at near 0% confluence.
*DAPI shows faint blue coloration in some cells, however no insulin or glucagon detected.
 




Latest revision as of 00:06, 27 September 2017

Cell Fate Switch by Synthetic Transcription Factors Main project page
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Immunocytology T1

Standard Procedure: Immunocytology

Antibodies Used:

Antibody Secondary/Primary Fluorophore Dilution Catalog
Glucagon (FL-180) Primary --- 1:500 sc-13091
Insulin Primary --- 1:250 ab7842
Goat Anti-Guinea Pig IgG Secondary Red-Alexaflour 594 1:1000 ab1501888
Rabbit Anti-Glucagon Secondary Green 1:1000 ---
Hoechst 33325 Secondary Blue 1:1000 ---

Results

  • Too few cells fixated for useful results. Plates were at near 0% confluence.
  • DAPI shows faint blue coloration in some cells, however no insulin or glucagon detected.



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