User:David K. Barclay/Notebook/Controlling Pancreas Cell Fate Using Transcription Factors/2014/06/23: Difference between revisions
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== | ==Bioinformatics- Promoter/H3K27me3 mark crosses in excel== | ||
===GALAXY Procedure=== | |||
# Download .bed data from lab's backfile and upload to GALAXY (usegalaxy.org). Toolbar left > Get Data > Upload File > Select .bed file > File Format: Interval, Genome: hg18 > Execute. | |||
# Upload refseq promoters (hg18) in same fashion as .bed data if stored on hard drive. | |||
# Intersect promoter with crosses: Toolbar left > Operate on Genomic Intervals > Intersect > Return: Overlapping Intervals, of: methyl-histone data, that intersect: promoters, for at least: 1 bp > Execute. | |||
# Once new data analysis is run, regions should display under title for analysis. Enter all crosses data into excel spreadsheet for analysis. | |||
# Intersect new cross data with all other crosses made. This will give regions that both cell experiments contain. For example, a cross for cell experiments A1 and B1 will contain all regions that have histone methylation in both data. | |||
===Excel Procedure=== | |||
# Create a table consisting of all cell experiments from alpha cells to exocrine cells on both the x and y axis (Figure 1). | |||
# In each cross (e.g. A1 cross B1) mark down the number of regions similar. Data will come from previous GALAXY crosses. | |||
# Heat map analysis using conditional formatting on Excel. Darker numbers = near maximum, lighter numbers = near minimum. | |||
* Crosses between H3K27me3 data (Bramswig et al. 13)and reference promoters were done in Excel. They show number of genes hypothetically controlled by the histone methylation mark. | |||
[[Image:Bramswig Crosses.png]] | |||
*Figure 1 | |||
*Crosses revealed around 4950 promoters were controlled in alpha cells. Heat map analysis indicates that alpha cells are more heavily affected by H3K27me3 marks than beta cells. Crosses between alpha/beta cell histone methylation (i.e. A1-3 cross B1-3 on the table) show there are around 1000 genes that are associated with the methyl-histone mark in alpha cells but not in bet cells. | |||
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Revision as of 12:24, 11 July 2014
Cell Fate Switch by Synthetic Transcription Factors | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Bioinformatics- Promoter/H3K27me3 mark crosses in excelGALAXY Procedure
Excel Procedure
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