User:David K. Barclay/Notebook/Chromatin State Sensor/2015/07/01: Difference between revisions
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== | ==David Barclay 07/01== | ||
'''Passaging - U2OS''' | |||
* Protocol: [http://openwetware.org/wiki/Haynes:SplittingCells Splitting Cells] | |||
* Cell culture medium is: McCoy's 5A, 10% FBS (tet-free), 1% P/S | |||
* Use 90-100% confluent '''T-25''' | |||
* Make 1 new T-75 at a 1:10 dilution | |||
* Passage number: 0 | |||
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Revision as of 12:55, 1 July 2015
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David Barclay 07/01Passaging - U2OS
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