User:David K. Barclay/Notebook/Chromatin State Sensor/2015/06/24: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==David Barclay - 06/24/15== | ||
* | |||
* Thawing - TC1alpha, split 2:8 | |||
* Passaging- U2OS 1:9 | |||
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'''Passaging - U2OS''' | |||
* Protocol: [http://openwetware.org/wiki/Haynes:SplittingCells Splitting Cells] | |||
* Cell culture medium is: McCoy's 5A, 10% FBS (tet-free), 1% P/S | |||
* Use 90-100% confluent '''T-25''' | |||
* Make 1 new T-75 at a 1:10 dilution | |||
* Passage number: 0 | |||
---- | |||
'''Thawing''' | |||
* Protocol: [http://openwetware.org/wiki/Haynes:ThawingCells Thawing Cells] | |||
* Cell culture medium is: DMEM, 20% FBS (tet-free), 1% P/S | |||
* From Frozen Cell Stocks (3 remaining) | |||
* Make 1 new T-25 | |||
* Passage number: alpha | |||
Latest revision as of 01:01, 27 September 2017
Project name | Main project page Previous entry Next entry |
David Barclay - 06/24/15
Passaging - U2OS
Thawing
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