User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/10/15: Difference between revisions

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==Remains of the Day==
==Remains of the Day==
* Write up your awesome plans and results here...
* Work on addressing Manish's comments
* Transforming ligations from Friday with Steph
* Waiting for results from Guelph to write up potato and organic amendment reports
* Work on handout for meeting on Friday
* Figure out RT PCR primers for strawberry experiment
** B. subtilis primers will be from here: http://apsjournals.apsnet.org.proxy1.lib.uwo.ca/doi/abs/10.1094/PHYTO-06-12-0123-R
** P. fluorescens primers will be from here: http://aem.asm.org/content/73/17/5531.full.pdf+html
** B. phytofirmans primers will be from here (picking endophytic genes and designing primers against those cause no one has B. phytofirmans primers): http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3639386/
 
* Interstingly, I just discovered FB2 is Pseudomonas aeruginosa strain FB2 (ACCESSION: HQ658762) rather than P. fluorescens. ;-\





Revision as of 14:03, 15 October 2013

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Remains of the Day

  • Interstingly, I just discovered FB2 is Pseudomonas aeruginosa strain FB2 (ACCESSION: HQ658762) rather than P. fluorescens. ;-\