User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/10/15

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(Autocreate 2013/10/15 Entry for User:David_Johnston_Monje/Notebook/Maize_Endophyte_Biofertilizers)
Current revision (16:03, 15 October 2013) (view source)
(Remains of the Day)
 
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==Remains of the Day==
==Remains of the Day==
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* Write up your awesome plans and results here...
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* Work on addressing Manish's comments
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* Transforming ligations from Friday with Steph
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* Waiting for results from Guelph to write up potato and organic amendment reports
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* Work on handout for meeting on Friday
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* Figure out RT PCR primers for strawberry experiment
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** B. subtilis primers will be from here: http://apsjournals.apsnet.org.proxy1.lib.uwo.ca/doi/abs/10.1094/PHYTO-06-12-0123-R
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** P. fluorescens primers will be from here: http://aem.asm.org/content/73/17/5531.full.pdf+html
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** B. phytofirmans primers will be from here (picking endophytic genes and designing primers against those cause no one has B. phytofirmans primers): http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3639386/
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* Interstingly, I just discovered FB2 is Pseudomonas aeruginosa strain FB2 (ACCESSION: HQ658762) rather than P. fluorescens. ;-\

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Applied Soil Microbial Ecology Main project page
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Remains of the Day

  • Interstingly, I just discovered FB2 is Pseudomonas aeruginosa strain FB2 (ACCESSION: HQ658762) rather than P. fluorescens. ;-\



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