User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/10/07: Difference between revisions
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==Remains of the Day== | ==Remains of the Day== | ||
* | * Figuring out clade specific TRFLP for the strawberries - Alpha proteobacteria (Methylobacteria), Beta proteobacteria (Burkholderia), Gamma proteobacteria (Pseudomonas) and Bacillus (Firmicutes) | ||
** Looking at several papers to research this TRFLP (http://aem.asm.org/content/71/7/4117/T1.expansion.html) - (http://www.ncbi.nlm.nih.gov/pubmed/17635538?dopt=Abstract&holding=npg) - (http://www.ncbi.nlm.nih.gov/pubmed/19554039) | |||
** Thinking on it more, it seems likely that real time PCR would be better. Want to try using Sbyr Green method for all, and compare to a general bacterial RT PCR. For P. fluorescencs this may be a start (http://aem.asm.org/content/72/4/2421.full), for B. subtilis (http://www.ncbi.nlm.nih.gov/pubmed/21356471) or (http://pubs.rsc.org.proxy1.lib.uwo.ca/en/content/articlehtml/2008/em/b806603e) and Burkholderia phytofirmans (http://www.plosone.org/article/info:doi/10.1371/journal.pone.0069435) -- this is not straightforward, gonna have to talk with Keri to see what we can do. | |||
** Strawberry microbial community profiling by DGGE - http://www.ncbi.nlm.nih.gov/pubmed/16629753?dopt=Abstract&holding=npg | |||
* Write up tree stuff to include in Kristin report | |||
Successful Fungal TRFLP from my small root rot symptoms the other day: | |||
[[image:Oct 7,2013Gel.tif]] | |||
* Beneficial PGPR - http://www.ncbi.nlm.nih.gov/pubmed/20635120 | |||
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Revision as of 16:27, 7 October 2013
 Applied Soil Microbial Ecology
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Remains of the Day
Successful Fungal TRFLP from my small root rot symptoms the other day:
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