User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/10/07: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 9: Line 9:
* Figuring out clade specific TRFLP for the strawberries - Alpha proteobacteria (Methylobacteria), Beta proteobacteria (Burkholderia), Gamma proteobacteria (Pseudomonas) and Bacillus (Firmicutes)
* Figuring out clade specific TRFLP for the strawberries - Alpha proteobacteria (Methylobacteria), Beta proteobacteria (Burkholderia), Gamma proteobacteria (Pseudomonas) and Bacillus (Firmicutes)
** Looking at several papers to research this TRFLP (http://aem.asm.org/content/71/7/4117/T1.expansion.html)  -  (http://www.ncbi.nlm.nih.gov/pubmed/17635538?dopt=Abstract&holding=npg)  -  (http://www.ncbi.nlm.nih.gov/pubmed/19554039)
** Looking at several papers to research this TRFLP (http://aem.asm.org/content/71/7/4117/T1.expansion.html)  -  (http://www.ncbi.nlm.nih.gov/pubmed/17635538?dopt=Abstract&holding=npg)  -  (http://www.ncbi.nlm.nih.gov/pubmed/19554039)
** Thinking on it more, it seems likely that real time PCR would be better. For P. fluorescencs this may be a start (http://aem.asm.org/content/72/4/2421.full), for B. subtilis ( )
** Thinking on it more, it seems likely that real time PCR would be better. Want to try using Sbyr Green method for all, and compare to a general bacterial RT PCR. For P. fluorescencs this may be a start (http://aem.asm.org/content/72/4/2421.full), for B. subtilis (http://www.ncbi.nlm.nih.gov/pubmed/21356471) or (http://pubs.rsc.org.proxy1.lib.uwo.ca/en/content/articlehtml/2008/em/b806603e) and Burkholderia phytofirmans (http://www.plosone.org/article/info:doi/10.1371/journal.pone.0069435) -- this is not straightforward, gonna have to talk with Keri to see what we can do.
** Strawberry microbial community profiling by DGGE - http://www.ncbi.nlm.nih.gov/pubmed/16629753?dopt=Abstract&holding=npg
* Write up tree stuff to include in Kristin report
* Write up tree stuff to include in Kristin report


* Beneficial PGPR - http://www.ncbi.nlm.nih.gov/pubmed/20635120


<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 15:42, 7 October 2013

Applied Soil Microbial Ecology <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Remains of the Day


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:David_Johnston_Monje/Notebook/Maize_Endophyte_Biofertilizers/2013/10/07&oldid=735818"