User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/10/07: Difference between revisions
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* Figuring out clade specific TRFLP for the strawberries - Alpha proteobacteria (Methylobacteria), Beta proteobacteria (Burkholderia), Gamma proteobacteria (Pseudomonas) and Bacillus (Firmicutes) | * Figuring out clade specific TRFLP for the strawberries - Alpha proteobacteria (Methylobacteria), Beta proteobacteria (Burkholderia), Gamma proteobacteria (Pseudomonas) and Bacillus (Firmicutes) | ||
** Looking at several papers to research this TRFLP (http://aem.asm.org/content/71/7/4117/T1.expansion.html) - (http://www.ncbi.nlm.nih.gov/pubmed/17635538?dopt=Abstract&holding=npg) - (http://www.ncbi.nlm.nih.gov/pubmed/19554039) | ** Looking at several papers to research this TRFLP (http://aem.asm.org/content/71/7/4117/T1.expansion.html) - (http://www.ncbi.nlm.nih.gov/pubmed/17635538?dopt=Abstract&holding=npg) - (http://www.ncbi.nlm.nih.gov/pubmed/19554039) | ||
** Thinking on it more, it seems likely that real time PCR would be better. For P. fluorescencs this may be a start (http://aem.asm.org/content/72/4/2421.full), for B. subtilis ( ) | |||
* Write up tree stuff to include in Kristin report | * Write up tree stuff to include in Kristin report | ||
Revision as of 09:18, 7 October 2013
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Remains of the Day
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