User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/08/09

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(Remains of the Day)
Current revision (17:14, 9 August 2013) (view source)
(Remains of the Day)
 
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* Streak out plates of P. syringae DC95-5 to inoculate tomato leaves with on Monday
* Streak out plates of P. syringae DC95-5 to inoculate tomato leaves with on Monday
* Run gels of nested Oomycete PCRs (looks like a good approach, but using experience from 2013/06/27, I should do 40 cycles, have more MgCl2 and use 57 degree annealing rather than 62)
* Run gels of nested Oomycete PCRs (looks like a good approach, but using experience from 2013/06/27, I should do 40 cycles, have more MgCl2 and use 57 degree annealing rather than 62)
 +
* Did 96 well PCR of all the Cali samples using DC6-OomR. Problem was that I dropped the plate and over half spilt, so I reallocated liquid so that there was only 10 ul per well, rather than 25. Hope it'll work well enough for nested PCR.
[[Image:August9,2013Gel.tif]]
[[Image:August9,2013Gel.tif]]

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Remains of the Day

  • Repeat tomato plant spray with Actigard and TBG products
  • Streak out plates of P. syringae DC95-5 to inoculate tomato leaves with on Monday
  • Run gels of nested Oomycete PCRs (looks like a good approach, but using experience from 2013/06/27, I should do 40 cycles, have more MgCl2 and use 57 degree annealing rather than 62)
  • Did 96 well PCR of all the Cali samples using DC6-OomR. Problem was that I dropped the plate and over half spilt, so I reallocated liquid so that there was only 10 ul per well, rather than 25. Hope it'll work well enough for nested PCR.

Image:August9,2013Gel.tif



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