User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/08/09: Difference between revisions
From OpenWetWare
| (2 intermediate revisions by the same user not shown) | |||
| Line 9: | Line 9: | ||
* Repeat tomato plant spray with Actigard and TBG products | * Repeat tomato plant spray with Actigard and TBG products | ||
* Streak out plates of P. syringae DC95-5 to inoculate tomato leaves with on Monday | * Streak out plates of P. syringae DC95-5 to inoculate tomato leaves with on Monday | ||
* Run gels of nested Oomycete PCRs | * Run gels of nested Oomycete PCRs (looks like a good approach, but using experience from 2013/06/27, I should do 40 cycles, have more MgCl2 and use 57 degree annealing rather than 62) | ||
* Did 96 well PCR of all the Cali samples using DC6-OomR. Problem was that I dropped the plate and over half spilt, so I reallocated liquid so that there was only 10 ul per well, rather than 25. Hope it'll work well enough for nested PCR. | |||
[[Image:August9,2013Gel.tif]] | |||
Revision as of 14:14, 9 August 2013
 Applied Soil Microbial Ecology
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Remains of the Day
| |
