User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/08/07: Difference between revisions
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==Remains of the Day== | ==Remains of the Day== | ||
* Put together some info for the Earth Alive project - strawberry and trees | * Put together some info for the Earth Alive project - strawberry and trees | ||
* Ran gel of the Oomycete PCR from yesterday - if it works, restrict with HhaI overnight and send in for TRFLP. Since it failed (only got primer dimers again) I'm gonna have to rerun the gradient PCR, since something is wrong here. | * Ran gel of the Oomycete PCR from yesterday - if it works, restrict with HhaI overnight and send in for TRFLP. Since it failed (only got primer dimers again) I'm gonna have to rerun the gradient PCR, since something is wrong here. Used P. capsici, California PSI Soil #9, California Pooled Dead Strawberries #23, and Dying arrugula rhizosphere. Used NEB buffer, 4 ul of 25 mM NEB MgCL, and the OomF and OomR primers. Ran the gradient on the Eppendorf machine, running 8 reactions over temperature range of 53 to 67. Doing 50 cycles, with 2 minute extention times. | ||
* Finished results section of the TVD fungi paper and sent to Steph and George for a look over | * Finished results section of the TVD fungi paper and sent to Steph and George for a look over | ||
* Call tree guys to find out status of the plants | * Call tree guys to find out status of the plants |
Revision as of 14:42, 7 August 2013
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Remains of the Day
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