User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/08/06: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 8: Line 8:
==Remains of the Day==
==Remains of the Day==
* Redo California strawberry oomycete PCRs, with lower annealing temperature (55 degrees) and more cycles - 50 perhaps vs 44. Also used the Invitrogen PCR buffer this time, instead of NEB which I used last time.   
* Redo California strawberry oomycete PCRs, with lower annealing temperature (55 degrees) and more cycles - 50 perhaps vs 44. Also used the Invitrogen PCR buffer this time, instead of NEB which I used last time.   
* Start treating tomato leaves with TBG products at previously ascertained concentrations.  
* Start treating Bonny Best tomato leaves with TBG products at previously ascertained concentrations.  


**1. Water control: deionized water was sprayed onto leaves until dripping
**1. Water control: deionized water was sprayed onto leaves until dripping

Revision as of 14:29, 6 August 2013

Applied Soil Microbial Ecology <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Remains of the Day

  • Redo California strawberry oomycete PCRs, with lower annealing temperature (55 degrees) and more cycles - 50 perhaps vs 44. Also used the Invitrogen PCR buffer this time, instead of NEB which I used last time.
  • Start treating Bonny Best tomato leaves with TBG products at previously ascertained concentrations.
    • 1. Water control: deionized water was sprayed onto leaves until dripping
    • 2. Actigard powder was mixed at 100 ug / mL and sprayed onto leaves until dripping,
    • 3. Fraction F4, at a 1:75 dilution, was pH adjusted to 7, then sprayed onto leaves until dripping
    • 4. Fraction FX, at a 1:75 dilution, was pH adjusted to 7, then sprayed onto leaves until dripping
    • 5. TBG liquid, at a 1:50 dilution, was sprayed onto leaves until dripping
  • Put together some info for the Earth Alive project - strawberry and trees



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:David_Johnston_Monje/Notebook/Maize_Endophyte_Biofertilizers/2013/08/06&oldid=714646"