User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/07/17: Difference between revisions
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==Remains of the Day== | ==Remains of the Day== | ||
* Ran last night's PCR testing 27F degen and 1492Rh primers on strawberry samples. All temperatures worked (see below) but it looks like 52 degree annealing might be the best. Gonna apply this to growth room trial 1 samples and run TRFLP digesting with DdeI - but will have Stephanie do this tommorow as she's conducted all the other strawberry trial PCRs. | * Ran last night's PCR testing 27F degen and 1492Rh primers on strawberry samples. All temperatures worked (see below) but it looks like 52 degree annealing might be the best. Gonna apply this to growth room trial 1 samples and run TRFLP digesting with DdeI - but will have Stephanie do this tommorow as she's conducted all the other strawberry trial PCRs. | ||
* Run OomF/OomR PCR on California PSI soil samples from January 21, 2013, alongside the sick arrugula root and rhizosphere samples, 5 Albion speedling rhizospheres grown on Brian's soil, and all | * Run OomF/OomR PCR on California PSI soil samples from January 21, 2013, alongside the sick arrugula root and rhizosphere samples, 5 Albion speedling rhizospheres grown on Brian's soil, and all 8 oomycete DNA samples we have (loaded in alphabetical order - Phytopthera capsici and Phytopthera infestans 23 - Pythium aphanidermatum, Pythium dissotocum, Pythium intermedium, Pythium irregulare, Pythium sylvaticum, Pythium ultimum) | ||
[[Image:July 17,2013Gel.tif]] | [[Image:July 17,2013Gel.tif]] | ||
Revision as of 13:47, 17 July 2013
 Applied Soil Microbial Ecology
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Remains of the Day
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