User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/06/27: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Applied Soil Microbial Ecology </span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Applied Soil Microbial Ecology </span>
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* Got colonies of the molasses rhizosphere and root clone PCR from day before yesterday. Steph will do colony PCR with M13 primers and sequence
* Got colonies of the molasses rhizosphere and root clone PCR from day before yesterday. Steph will do colony PCR with M13 primers and sequence
* Starting to get leaf speck symptoms on the Bonny Best tomatoes. Steph will hopefully monitor and photograph in the next few days.
* Starting to get leaf speck symptoms on the Bonny Best tomatoes. Steph will hopefully monitor and photograph in the next few days.
* Oomycete TRFLP primers work great! Steph will hopefully continue to test them in my absensce (seee below)
* Oomycete TRFLP primers work great! Problem with P. ultimum reaction however, as I was looking for a 908 bp fragment there. Steph will hopefully continue to test them in my absensce (and she did on July 2 - see annotated gel on that date)


[[image: June 26,2014Gel.tif]]
[[image:June 26,2013Gel.tif]]




Latest revision as of 22:52, 26 September 2017

Applied Soil Microbial Ecology Main project page
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Remains of the Day

  • Got colonies of the molasses rhizosphere and root clone PCR from day before yesterday. Steph will do colony PCR with M13 primers and sequence
  • Starting to get leaf speck symptoms on the Bonny Best tomatoes. Steph will hopefully monitor and photograph in the next few days.
  • Oomycete TRFLP primers work great! Problem with P. ultimum reaction however, as I was looking for a 908 bp fragment there. Steph will hopefully continue to test them in my absensce (and she did on July 2 - see annotated gel on that date)



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