User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/06/27

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(Autocreate 2013/06/27 Entry for User:David_Johnston_Monje/Notebook/Maize_Endophyte_Biofertilizers)
Current revision (10:29, 17 July 2013) (view source)
(Remains of the Day)
 
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==Remains of the Day==
==Remains of the Day==
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* Write up your awesome plans and results here...
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* Got colonies of the molasses rhizosphere and root clone PCR from day before yesterday. Steph will do colony PCR with M13 primers and sequence
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* Starting to get leaf speck symptoms on the Bonny Best tomatoes. Steph will hopefully monitor and photograph in the next few days.
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* Oomycete TRFLP primers work great! Problem with P. ultimum reaction however, as I was looking for a 908 bp fragment there. Steph will hopefully continue to test them in my absensce (and she did on July 2 - see annotated gel on that date)
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[[image:June 26,2013Gel.tif]]

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Remains of the Day

  • Got colonies of the molasses rhizosphere and root clone PCR from day before yesterday. Steph will do colony PCR with M13 primers and sequence
  • Starting to get leaf speck symptoms on the Bonny Best tomatoes. Steph will hopefully monitor and photograph in the next few days.
  • Oomycete TRFLP primers work great! Problem with P. ultimum reaction however, as I was looking for a 908 bp fragment there. Steph will hopefully continue to test them in my absensce (and she did on July 2 - see annotated gel on that date)

image:June 26,2013Gel.tif



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