User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/04/17: Difference between revisions
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==Remains of the Day== | ==Remains of the Day== | ||
* | * Measured OD600s of the bacterial spot and speck grown overnight in R2A and adjusted it to around 0.12 for all of them. Diluted them to 10^-5, 10^-6, 10^-7,10^-8 and plated 25 ul of each on a quadrant of an R2A plate. | ||
* After reading a paper about bacterial primer testing (Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies) I found website called SILVA for testing bacterial primers - http://www.arb-silva.de/search/testprime/ | |||
* Found paper about Burkholderia specific primers: (Molecular Method To Assess the Diversity of Burkholderia Species in Environmental Samples) | |||
* Found paper about Pseudomonas specific primers: (An rpoD-based PCR procedure for the identification of Pseudomonas species and for their detection in environmental samples.) | |||
* George showed me a great TRFLP assignment website for the program called PAT: https://secure.limnology.wisc.edu/trflp/index.jsp | |||
Revision as of 15:11, 17 April 2013
 Applied Soil Microbial Ecology
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Remains of the Day
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