User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/04/12

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(Remains of the Day)
Current revision (16:22, 12 April 2013) (view source)
(Remains of the Day)
 
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==Remains of the Day==
==Remains of the Day==
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* Paint bacterial speck and spot suspensions onto plants and incubate for two weeks.
+
* Paint bacterial speck and spot suspensions onto plants and incubate for two weeks. - did not do, perhaps Tuesday when other true leaves start to emerge
 +
* Sequences failed cause the M13 Forward primer falls outside the amplified region. Must use T7 or SP6 to sequence.
 +
* Made virtual TRFLP for individual strains used  in the strawberry innoculation experiment. Only enterobacter are really detectable with those primers. :-/

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Remains of the Day

  • Paint bacterial speck and spot suspensions onto plants and incubate for two weeks. - did not do, perhaps Tuesday when other true leaves start to emerge
  • Sequences failed cause the M13 Forward primer falls outside the amplified region. Must use T7 or SP6 to sequence.
  • Made virtual TRFLP for individual strains used in the strawberry innoculation experiment. Only enterobacter are really detectable with those primers. :-/



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