User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/04/10: Difference between revisions
From OpenWetWare
(fix raw html notebook nav) |
|||
(7 intermediate revisions by one other user not shown) | |||
Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Applied Soil Microbial Ecology </span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Applied Soil Microbial Ecology </span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
==Remains of the Day== | ==Remains of the Day== | ||
* | * Using the same liquid cultures as before (bad idea as the bacteria might have been dead) I redid the plating assay for the bacterial spot and speck onto LB plates at 10^-7, 10^-8, 10^-9, 10^-10 concentrations (25 ul of each on a quarter plate). Got the OD600s for the 10^-1 dilutions (50 ul of TSB in 450 ul of sterile buffer) and they are as follows: Pst T601=0.372, Pst DC95=0.267, Pst DC08=0.297, Pst DC01=0.357, Xg 444=0.369, Xg 1BSB=0.414, Xg DC03=0.036, Xg DC00=0.094. Got the OD600s for the 10^-2 dilutions (10 ul of the 10^-1 dilution in 90 ul of sterile buffer) and they are as follows: Pst T601=0.034, Pst DC95=0.021, Pst DC08=0.028, Pst DC01=0.033, Xg 444=0.037, Xg 1BSB=0.037, Xg DC03=0.004, Xg DC00=0.012. Two days later, Xg 444 and Xg 1B5B had colonies - (Xg 444 at 10^-7=52, 10^-8=7) and (Xg 1B5B at 10^-7=11, 10^-8=5). Three days later, the Xg DC03 at 10^-4 produced 27 colonies (none at higher dilutions). | ||
* Stephanie sent in all the sequencing, including my most recent set of colony PCRs for the physical capture TRFLP clones. | |||
Latest revision as of 22:37, 26 September 2017
Applied Soil Microbial Ecology | Main project page Previous entry Next entry |
Remains of the Day
|