User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/04/09

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(Remains of the Day)
(Remains of the Day)
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* Measure spot and speck CFUs by plating (4 half plates will be used) with 50 ul of 10^-3, 10^-4, 10^-5, and 10^-6 --> compare to OD600 values
* Measure spot and speck CFUs by plating (4 half plates will be used) with 50 ul of 10^-3, 10^-4, 10^-5, and 10^-6 --> compare to OD600 values
* Run gel of physical capture TRFLP PCRs and send the lot of them for sequencing after specing the DNA
* Run gel of physical capture TRFLP PCRs and send the lot of them for sequencing after specing the DNA
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[[image:April 9,2013Gel.tif]]

Revision as of 14:11, 9 April 2013

Applied Soil Microbial Ecology Main project page
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Remains of the Day

  • Measure spot and speck CFUs by plating (4 half plates will be used) with 50 ul of 10^-3, 10^-4, 10^-5, and 10^-6 --> compare to OD600 values
  • Run gel of physical capture TRFLP PCRs and send the lot of them for sequencing after specing the DNA

image:April 9,2013Gel.tif



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