User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/03/21: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2013/03/21 Entry for User:David_Johnston_Monje/Notebook/Maize_Endophyte_Biofertilizers)
 
(fix raw html notebook nav)
 
(One intermediate revision by one other user not shown)
Line 2: Line 2:
|-
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Applied Soil Microbial Ecology </span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Applied Soil Microbial Ecology </span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
|-
|-
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Remains of the Day==
==Remains of the Day==
* Write up your awesome plans and results here...
* Innoculated the preped nodal potatoes with bacteria - resuspended 1 freshly grown colony from glycerol stock, into 500 ul of sterile 0.1 M sodium phosphate and then to each of 3 potatoes, added 100 ul of the suspension. Went backwards, starting with 12H and making it all the way to 7A - a few more will get done tommorow.  
* Started physical capture TRFLP for 5 growth room Heinz 2401 rhizospheres and 5 Heinz 2401 field roots - digesting them overnight at 60 degrees with BstNI.  




Latest revision as of 22:34, 26 September 2017

Applied Soil Microbial Ecology Main project page
Previous entry      Next entry

Remains of the Day

  • Innoculated the preped nodal potatoes with bacteria - resuspended 1 freshly grown colony from glycerol stock, into 500 ul of sterile 0.1 M sodium phosphate and then to each of 3 potatoes, added 100 ul of the suspension. Went backwards, starting with 12H and making it all the way to 7A - a few more will get done tommorow.
  • Started physical capture TRFLP for 5 growth room Heinz 2401 rhizospheres and 5 Heinz 2401 field roots - digesting them overnight at 60 degrees with BstNI.



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:David_Johnston_Monje/Notebook/Maize_Endophyte_Biofertilizers/2013/03/21&oldid=1012250"