Ran gel of yesterday's fungal PCR (those with stars beside the name will be repeated with 40 cycles/TVD fields will all be repeated with 40):
None of the replacement PCRs that I did for day before yesterday's bacterial TRFLP worked. This time, repeating with 50 ul reaction volume and 5 extra cycles (as well as for all failed fungal reactions and all TVD field fungal).
Got Steph started on culturing microbes for the strawberry rhizosphere experiment.
Extracted 23 more TVD growth room rep 2 rhizospheres