User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/02/05

Current revision

Applied Soil Microbial Ecology

Interviewed intern candidates most of the day
Did NifH primer test, as gradient from 52 to 62 degrees, with 40 cycles, 2.5 minutes extension, 25 ul of reaction and Klebsiella pneumonii as a positive control, E. coli as negative, Methylobacterial cells as test and corn soil DNA as a test.
Did Oomycete TRFLP primer test, as gradient from 52 to 62 degrees, with 40 cycles, 2.5 minutes extension, 25 ul of reaction and infected arrugula rhizosphere as a positive control, and Alliston potato soil DNA as a test.
Autoclaved LGI broth for use in plate incubation test tommorow.
Did PCR for fungal trflp of California soils that Joseph extracted since he's been having a hard time doing it.

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 *Did Oomycete TRFLP primer test, as gradient from 52 to 62 degrees, with 40 cycles, 2.5 minutes extension, 25 ul of reaction and infected arrugula rhizosphere as a positive control,  and Alliston potato soil DNA as a test.
 *Autoclaved LGI broth for use in plate incubation test tommorow.
+* Did PCR for fungal trflp of California soils that Joseph extracted since he's been having a hard time doing it.