User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/01/15: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2013/01/15 Entry for User:David_Johnston_Monje/Notebook/Maize_Endophyte_Biofertilizers)
 
(fix raw html notebook nav)
 
(2 intermediate revisions by one other user not shown)
Line 2: Line 2:
|-
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Applied Soil Microbial Ecology </span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Applied Soil Microbial Ecology </span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
|-
|-
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Remains of the Day==
==Remains of the Day==
* Write up your awesome plans and results here...
* Sent George the maize TBG report yesterday
* reviewing the biocontrol chapter one last time - did it and George sent it off
* pouring R2A plates to begin isolating microbes - using phone as portable photo display to help select colonies (poured plates, will start isolating tommorow)
* Ran two PCRs for enzyme trial of TRFLP - 30 cycles, 56 degree annealing with 3 seventy five mL reactions with sample 1 (alliston soil) and #1 - 17 of Mel's samples. This was repeated for fungal TRFLP, but with 35 cycles and 48 degree annealing. Will test purification or not, with HhaI, DdeI and BstNI cuts. (two different companies of HhaI for the bacteria)




Latest revision as of 22:21, 26 September 2017

Applied Soil Microbial Ecology Main project page
Previous entry      Next entry

Remains of the Day

  • Sent George the maize TBG report yesterday
  • reviewing the biocontrol chapter one last time - did it and George sent it off
  • pouring R2A plates to begin isolating microbes - using phone as portable photo display to help select colonies (poured plates, will start isolating tommorow)
  • Ran two PCRs for enzyme trial of TRFLP - 30 cycles, 56 degree annealing with 3 seventy five mL reactions with sample 1 (alliston soil) and #1 - 17 of Mel's samples. This was repeated for fungal TRFLP, but with 35 cycles and 48 degree annealing. Will test purification or not, with HhaI, DdeI and BstNI cuts. (two different companies of HhaI for the bacteria)



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:David_Johnston_Monje/Notebook/Maize_Endophyte_Biofertilizers/2013/01/15&oldid=1011757"