User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2012/12/14

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(Autocreate 2012/12/14 Entry for User:David_Johnston_Monje/Notebook/Maize_Endophyte_Biofertilizers)
Current revision (20:10, 14 December 2012) (view source)
(Remains of the Day)
 
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==Remains of the Day==
==Remains of the Day==
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* Write up your awesome plans and results here...
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* Skipped doing stats on the tree soil data, and photographed and mashed up one of each potato genotype for follow up culturing experiment. Replaced the 2 bleach, 1 ethanol wash with one 10 minute wash with 0.005% Triton X-100 (50 ul in 1 L of water) and one 15 minute was in 1/10 bleach wash plus TritonX. This was followed by 3 washes in autoclaved water. Diluted the samples in buffer as before after Kleco machine homogenization and then left them in 4 degree overnight til tommorow when I plate em.
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Remains of the Day

  • Skipped doing stats on the tree soil data, and photographed and mashed up one of each potato genotype for follow up culturing experiment. Replaced the 2 bleach, 1 ethanol wash with one 10 minute wash with 0.005% Triton X-100 (50 ul in 1 L of water) and one 15 minute was in 1/10 bleach wash plus TritonX. This was followed by 3 washes in autoclaved water. Diluted the samples in buffer as before after Kleco machine homogenization and then left them in 4 degree overnight til tommorow when I plate em.


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