User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2012/11/27

Current revision

Applied Soil Microbial Ecology

Making glycerol stocks of rhizospheres, roots and shoots for potato endophyte experiment - shoots are unlickley to be interesting but do them anyway.
After culturing and making glycerol stocks, freeze the falcon tubes for later DNA extraction
Weighed mash collected, and added twice as much phosphate buffer to each (ie. 1 g of mash recieved 2 mL). Plating roots (50 ul of 100X diluted mash each third of a genotype plate) and shoots (just the 10X dilution) on plates divided into 3 and grouped by genotype. Also innoculating the into semi-solid LGI (50 ul of 10X diluted stuff for shoot, 50 ul of 100X diluted stuff for roots).

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 * Making glycerol stocks of rhizospheres, roots and shoots for potato endophyte experiment - shoots are unlickley to be interesting but do them anyway.
 * After culturing and making glycerol stocks, freeze the falcon tubes for later DNA extraction
-* Weighed mash collected, and added twice as much phosphate buffer to each (ie. 1 g of mash recieved 2 mL). Plating roots (50 ul of 10X andd 100X diluted mash each third of a genotype plate) and shoots (just the 10X dilution) on plates divided into 3 and grouped by genotype. Also innoculating the 10X diluted stuff into semi-solid LGI.
+* Weighed mash collected, and added twice as much phosphate buffer to each (ie. 1 g of mash recieved 2 mL). Plating roots (50 ul of 100X diluted mash each third of a genotype plate) and shoots (just the 10X dilution) on plates divided into 3 and grouped by genotype. Also innoculating the into semi-solid LGI (50 ul of 10X diluted stuff for shoot, 50 ul of 100X diluted stuff for roots).
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