User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2012/11/26 - Revision history

David Johnston Monje: /* Remains of the Day */

David Johnston Monje — Tue, 27 Nov 2012 01:51:16 GMT

Remains of the Day

←Older revision		Revision as of 01:51, 27 November 2012
Line 9:		Line 9:
	* Today is a day for surface photographing, weighing, surface sterilizing, grinding and culturing of endophytes from my potatoes downstairs. Spreading 10X and 100X dilutions on the plate for culturing, one plate per tissue (shoot or root), one per rep. Doing one genotype at a time so as to not have samples sitting around festering.		* Today is a day for surface photographing, weighing, surface sterilizing, grinding and culturing of endophytes from my potatoes downstairs. Spreading 10X and 100X dilutions on the plate for culturing, one plate per tissue (shoot or root), one per rep. Doing one genotype at a time so as to not have samples sitting around festering.
	** What actually happened is that I did Kennebec, Shepody, and HAW 1528 (rhizospheres, roots, and shoots). These were surface sterilized as with corn, but I didn't have autoclaved water to rinse the ethanol off at the end and instead drip dried the samples before homogenizing them individually for 20 seconds in the Kleco shaker (with 70% ethanol washing in between). I added 1 mL of sodium phosphate buffer to whatever semi-solid macerate was recovered in a 15 mL falcon tube, and did not have time to do more so I refridgerated these overnight for processing tommorow.		** What actually happened is that I did Kennebec, Shepody, and HAW 1528 (rhizospheres, roots, and shoots). These were surface sterilized as with corn, but I didn't have autoclaved water to rinse the ethanol off at the end and instead drip dried the samples before homogenizing them individually for 20 seconds in the Kleco shaker (with 70% ethanol washing in between). I added 1 mL of sodium phosphate buffer to whatever semi-solid macerate was recovered in a 15 mL falcon tube, and did not have time to do more so I refridgerated these overnight for processing tommorow.
		+	* Cool coincidence - a rep from Waters (Michel Shilling) came in to look around and mentioned the name Allan Sargent - he's apparently still around London, doing dairy research and flies a cessna airplane out of London airport.

David Johnston Monje: /* Remains of the Day */

David Johnston Monje — Tue, 27 Nov 2012 01:29:12 GMT

Remains of the Day

←Older revision		Revision as of 01:29, 27 November 2012
Line 8:		Line 8:
	==Remains of the Day==		==Remains of the Day==
	* Today is a day for surface photographing, weighing, surface sterilizing, grinding and culturing of endophytes from my potatoes downstairs. Spreading 10X and 100X dilutions on the plate for culturing, one plate per tissue (shoot or root), one per rep. Doing one genotype at a time so as to not have samples sitting around festering.		* Today is a day for surface photographing, weighing, surface sterilizing, grinding and culturing of endophytes from my potatoes downstairs. Spreading 10X and 100X dilutions on the plate for culturing, one plate per tissue (shoot or root), one per rep. Doing one genotype at a time so as to not have samples sitting around festering.
		+	** What actually happened is that I did Kennebec, Shepody, and HAW 1528 (rhizospheres, roots, and shoots). These were surface sterilized as with corn, but I didn't have autoclaved water to rinse the ethanol off at the end and instead drip dried the samples before homogenizing them individually for 20 seconds in the Kleco shaker (with 70% ethanol washing in between). I added 1 mL of sodium phosphate buffer to whatever semi-solid macerate was recovered in a 15 mL falcon tube, and did not have time to do more so I refridgerated these overnight for processing tommorow.

David Johnston Monje: /* Remains of the Day */

David Johnston Monje — Mon, 26 Nov 2012 14:48:09 GMT

Remains of the Day

←Older revision		Revision as of 14:48, 26 November 2012
Line 7:		Line 7:
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
	==Remains of the Day==		==Remains of the Day==
-	* Today is a day for surface photographing, weighing, surface sterilizing, grinding and culturing of endophytes from my potatoes downstairs	+	* Today is a day for surface photographing, weighing, surface sterilizing, grinding and culturing of endophytes from my potatoes downstairs. Spreading 10X and 100X dilutions on the plate for culturing, one plate per tissue (shoot or root), one per rep. Doing one genotype at a time so as to not have samples sitting around festering.

David Johnston Monje: /* Remains of the Day */

David Johnston Monje — Mon, 26 Nov 2012 14:42:39 GMT

Remains of the Day

←Older revision		Revision as of 14:42, 26 November 2012
Line 7:		Line 7:
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
	==Remains of the Day==		==Remains of the Day==
-	* ~~Write up your awesome plans~~ and ~~results here...~~	+	* Today is a day for surface photographing, weighing, surface sterilizing, grinding and culturing of endophytes from my potatoes downstairs

David Johnston Monje: Autocreate 2012/11/26 Entry for User:David_Johnston_Monje/Notebook/Maize_Endophyte_Biofertilizers

David Johnston Monje — Mon, 26 Nov 2012 14:41:30 GMT

Autocreate 2012/11/26 Entry for User:David_Johnston_Monje/Notebook/Maize_Endophyte_Biofertilizers

New page

{|{{table}} width="800"
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Applied Soil Microbial Ecology </span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
| colspan="2"|

==Remains of the Day==
* Write up your awesome plans and results here...


|}

__NOTOC__