User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2012/05/18

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Remains of the Day

  • Analyze strawberry rhizosphere TRFLP data that came in
  • Have Andrew look for strip lids to order for 96 well PCR
  • Plant small TBG corn germination trial for doing TRFLP (for George) - 5 plants per treatment
  • Write/plan for little potato culturing trial
  • Put additional strains from Guelph into glycerol stock here at A&L


Well # Species/Strain Plasmid Original freezing date
1 E. coli DH5alpha pDRIVE (empty) 2008
2 E. coli DH5alpha pDRIVE (Arabad biobricked promoter) 2008
3 E. coli DH5alpha pDRIVE (GFPuv biobricked) 2008
4 E. coli DH5alpha pCAMBIA 1305.2 (empty) 2008
5 E. coli DH5alpha pART27 (empty) 2008
6 E. coli DH5alpha pHANNIBAL (empty) 2008
7 E. coli JM109 pUC-EUY (something to do with iGEM) 2008
8 E. coli DH5alpha pCAMBIA 1305.2 (PopCTL1 promoter::GUS) 2008
9 E. coli DH5alpha Taq enzyme in unknown plasmid with Amp/Carb resistance 2008
10 Agrobacterium tumefascians GV3101 pCAMBIA 1305.2 (35S::PopCTL2) 2008
11 Agrobacterium tumefascians GV3101 pCAMBIA 1305.2 (PopCTL1 promoter::GUS) 2008
12 Klebsiella pneumonii 342 none 2008
13 Agrobacterium tumefascians GV3101 none 2008
14 E. coli JM109 none 2008
15 Pseudomonas putida UW4 none (empty) 2008
16 Bacilus subtilis GB03 none 2008
17 E. coli Nissle 1917 none 2008
18 Gluconacetobacter azotocaptans DS1 none 2008
19 Azospirrilum brasilensis SP7 none 2008
20 Azospirrilum brasilensis SP7 8.09.92 none 2008
21 Azospirrilum brasilensis SP7 NifA mutant none 2008
22 Azospirrilum brasilensis SP7 NifD mutant none 2008
23 Acetobacter diazotrophicus Nif mutant MAd3A (jar) none 2008
24 Acetobacter diazotrophicus Nif mutant MAd3A (plate) none 2008
25 Agrobacterium tumefascians GV3101 none 2008
26 Burkholderia phytofirmans PsJN none 2008
27 Mixteco maize isolated Burkholderia phytofirmans (3F9) none 2008
28 Gluconacetobacter azotocaptans DS1 none 2008
29 Bacillus subtilus GB03 (from Paul Pare) none 2009
30 Azospirrillum diazotrophicus Pal5 (from plate) none 2007
31 Azospirrillum diazotrophicus Pal5 (from broth) none 2007
32 E. coli Nissle 1917 pDSK-GFPuv 2008
33 E. coli Nissle 1917 AraC::crtE,B,I,Y::GFP in pSB1A2 2008
34 E. coli DH5alpha pSB1A2 2008
35 E. coli DH5alpha AraC::crtE,B,I,Y::GFP in pSB1A2 2008
36 E. coli DH5alpha pTG262 (broad host range plasmid) 2008
37 E. coli DH5alpha PSBA promoter in pDRIVE 2008
38 Electrocompetent Kp342 none 2008
39 Electrocompetent P. agglomerans (1A1) none 2008
40 Electrocompetent P. agglomerans (1A1) none 2008



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