User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2012/03/09

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Remains of the Day

  • Do real time PCR on potato cDNA samples from Jan 18 that were made yesterday
  • Qiagen RNeasy kit arrived - use it next week
  • Begin all soil treatments (weight assumed to be 200 kg/m^2 - all % mixtures are weight or volume product:weight dry soil - amendmants will be applied in 1 L of water mixed with fertilizer to 1 kg of soil) for tomato amendmant rhizosphere trial including treatments with 5 plants each
    • Autoclaved sand
    • Negative control (just water and initial fertilization)
    • Pasteurized (75 degree oven for 12 hours)
    • Terrabiogen liquid 0.01%
    • Terrabiogen liquid 0.01% + Terrabiogen solid 0.025%
    • Terrabiogen solid 0.025%
    • Biofence brassica meal 0.1%
    • OmegaGrow Fish Emulsion 0.5% (based on volume)
    • Molasses 1% (based on volume)
    • Azospirrilum and Strenotrophomonas dip and drench (each at OD600=0.2 in water)

Terrabiogen liquid appears to have been applied previously at 0.0000002347 mL per kg of soil (half strength in TVD report). I will apply at 0.1 mL per kg then. Terrabiogen solid appears to have been applied previously at 0.25 g per kg of soil (full strength in TVD report). I will apply at 0.25 mL per kg then.

(to be clear, 1% means I'm using 10 g product/kg of soil, or 10 mL of product per kg of soil)


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